LongAmp Hot Start Taq 2X Master Mix
NEB M0533L

LongAmp Hot Start Taq 2X Master Mix – 500 reactions; Storage Temp: -20°C; Shipping: Cool Packs; UN Code: 0000

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LongAmp Hot Start Taq 2X Master Mix contains a unique blend of aptamer-based Hot Start Taq and Deep Vent® DNA Polymerases. The aptamer-based inhibitor binds reversibly to the enzyme, inhibiting polymerase activity at temperatures below 45°C, but releases the enzyme during normal PCR cycling conditions. This permits assembly of PCR reactions at room temperature. The LongAmp® Hot Start Taq 2X Master Mix does not require a separate high temperature incubation step to activate the enzyme. The 3´→5´ exonuclease activity of Deep Vent DNA Polymerase increases the fidelity and robust amplification of Hot Start Taq DNA Polymerase (1). LongAmp® Hot Start Taq DNA Polymerase offers two-fold higher fidelity than Hot Start Taq DNA Polymerase alone. The convenient master mix formulation is supplied at a 2X concentration and contains dNTPs and Mg++, requiring only the addition of primers and DNA template for robust amplification. A wide range of PCR products can be generated; up to 30 kb from lambda or human genomic DNA.

Amplification of a 8 kb amplicon from varying amounts of Jurkat genomic DNA using LongAmp Hot Start Taq 2X Master Mix. Starting template amounts are indicated below the gel. Marker M is the NEB 2-Log DNA Ladder (NEB #N3200 ).

Product Source

An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1 and an E. coli strain that carries the Deep Vent® DNA Polymerase gene from Pyrococcus species GB-D.

Unit Definition:
One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 75°C.

Unit Assay Conditions:
1X ThermoPol™ Reaction Buffer, 200 µM dNTPs including [3H]-dTTP and 200 µg/ml activated Calf Thymus DNA.

This product is related to the following categories:
LongAmp® DNA Polymerases Products, 
Master Mixes Products
This product can be used in the following applications:
Hot Start PCR, 
Long Range PCR, 
Multiplex PCR,

Specialty PCR, Routine PCR, PCR

  • Reagents Supplied

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