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<b>Murine RNase Inhibitor</b> <sub><h6>NEB M0314L</h6></sub>

Murine RNase Inhibitor
NEB M0314L

“urine RNase Inhibitor, 15000 units;
Storage Temp: -20°C; Shipping: Cool
Packs; UN Code: 0000”

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RNase Inhibitor, Murine is a 50 kDa recombinant protein of murine origin. The inhibitor specifically inhibits RNases A, B and C. It inhibits RNases by binding noncovalently in a 1:1 ratio with high affinity. It is not effective against RNase 1, RNase T1, S1 Nuclease, RNase H or RNase from Aspergillus. In addition, no inhibition of polymerase activity is observed when RNase Inhibitor is used with Taq DNA Polymerase, AMV or M-MuLV Reverse Transcriptases, or Phage RNA Polymerases (SP6, T7, or T3).Recombinant murine RNase inhibitor does not contain the pair of cysteines identified in the human version that is very sensitive to oxidation, which causes inactivation of the inhibitor (1). As a result, RNase Inhibitor, Murine has significantly improved resistance to oxidation compared to the human/porcine RNase inhibitors, and is stable at low DTT concentrations (less than 1 mM). This makes it ideal for reactions where high concentration DTT is adverse to the reaction (eg. Real-time RT-PCR).

Product Source

An E. coli strain that carries the Ribonuclease Inhibitor gene from mouse.

This product is related to the following categories:
RNase Inhibitors, 
PURExpress, 
Cell-Free Protein Expression Products,

cDNA Synthesis & Reverse Transcriptases Products, RNA Synthesis In vitro Transcription (IVT), Protein Expression Products

This product can be used in the following applications:
NEBExpress® Cell-free E. coli Protein Synthesis System, 
PURExpress, 
RNA Cloning,

Cell-Free Protein Expression, Protein Expression, PCR

  • Reagents Supplied

    The following reagents are supplied with this product:

    NEB # Component Name Component # Stored at (°C) Amount Concentration
    • M0314S -20
      RNase Inhibitor, Murine M0314SVIAL -20 1 x 0.075 ml 40,000 units/ml
    • M0314L -20
      RNase Inhibitor, Murine M0314LVIAL -20 1 x 0.375 ml 40,000 units/ml

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