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<b>NEBNext® FFPE DNA Repair Mix </b> <sub><h6>NEB M6630L </h6></sub>

NEBNext® FFPE DNA Repair Mix
NEB M6630L

NEBNext FFPE DNA Repair Mix – 96 reactions; Storage Temp: -20°C; Shipping: Cool Packs; UN Code: 0000

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The NEBNext FFPE DNA Repair Mix contains enzymes and buffers that are optimized to repair FFPE DNA in next generation sequencing workflow. The mix can be used upstream of any next generation sequencing library construction method, for any sequencing platform.

Table 1: Types of FFPE DNA damage and their ability to be repaired by the NEBNext FFPE DNA Repair Mix

FFPE DAMAGE TYPE REPAIRED BY THE FFPE DNA ENZYME REPAIR MIX?
Deamination of cytosine to uracil Yes
Nicks and gaps Yes
Oxidized bases Yes
Blocked 3´ ends Yes
DNA fragmentation No
DNA-protein crosslinks No
Figure 1: FFPE DNA repair increases NGS library yields
An example of Agilent Bioanalyzer® traces of libraries prepared from stomach tumor FFPE DNA that was treated with the FFPE DNA Repair Mix, or was untreated, before library construction. Yield improvements of 10% to 458% have been observed. 
Figure 2. FFPE DNA repair increases NGS library yields 
FFPE DNA samples (25 ng or 50 ng) from a variety of tissue types were treated with the NEBNext FFPE DNA Repair Mix, or were untreated, before library construction. Library yields were measured using the Agilent Bioanalyzer, and ratios of library yields with and without treatment with the NEBNext FFPE DNA Repair Mix were calculated. Yield improvements with these samples ranged from 101% to 458%. 
Figure 3. FFPE DNA Repair improves sequence quality from FFPE samples and does not introduce new mutations 
Genomic DNA was extracted using standard DNA purification. Each DNA sample was treated with the NEBNext FFPE DNA Repair Mix, or was untreated, before library construction and Illumina® sequencing.
Panel A: The effect of DNA repair on the percentage of mappable reads. Reads were mapped to the human genome (hg19) using BWA (1).
Panel B: The effect of DNA repair on the percentage of reads that were properly paired from a paired-end sequencing protocol.for the full information click here

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