NEBNext Ultra II End Repair/dATailing Module
NEB E7546L

NEBNext Ultra II End Repair/dATailing Module – 96 reactions; Storage Temp: -20°C; Shipping: Cool Packs; UN Code: 0000

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The NEBNext Ultra II End Repair/dA-Tailing Module has been optimized to convert 500 pg-1 μg of fragmented DNA to repaired DNA having 5′ phosphorylated, 3′ dA-tailed ends. The module is optimized for use with the NEBNext Ultra II Ligation Module (NEB #E7595), and is part of the Ultra II DNA workflow which enables high yield preparation of high quality libraries from 500 pg to 1 µg of input DNA.

This module is compatible with Illumina workflows and with several Oxford Nanopore Technologies workflows.

For Illumina library construction, the NEBNext Ultra II End Repair/dA-Tailing Module is designed for use with the following:

• NEBNext Ultra II Ligation Module (NEB #E7595)
• NEBNext Ultra II Q5® Master Mix (NEB #M0544)
• NEBNext Oligos for Illumina (NEB #E7335#E7500#E7710#E7730#E6609#E7600 #E7535#E7350)

For use with NEBNext Multiplex Oligos for Illumina (Unique Dual Index UMI Adaptors DNA Set 1) (NEB #E7395), refer to the Protocols tab for UMI Adaptors-specific guidance.

Functional Validation

Each set of reagents is functionally validated together through construction and sequencing of a transcriptome library and sequenced on a DNA Library on the Illumina Sequencing Platform,

Lot Control

The lots provided are managed separately and qualified by additional functional validation. Individual reagents undergo standard enzyme activity and quality control assays, and also meet stringent criteria in the additional quality controls listed on each individual component page

This product is related to the following categories:
DNA Library Prep for Illumina, 
RNA Library Prep for Illumina, 
Next Generation Sequencing Library Preparation Products
  • Reagents Supplied

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